PHYTOCHEMICAL PROFILE AND INVITRO ANTIBACTERIAL ACTIVITY OF TRIDAX PROCUMBENS LEAF EXTRACT ON MULTI-DRUG-RESISTANT E. COLI AND S. AUREUS CLINICAL ISOLATES

Abstract

Tridax procumbens (family Asteraceae) is one of the medicinally important plants. T. procumbens was evaluated for various phytochemicals and antibacterial activity in this work. Isolation and identification of multi-drug-resistant (MDR) Staphyloccus aureus and Eschericia coli was evaluated. Antibiotic susceptibility pattern of patients attending selected health facilities in Makurdi was determined using ten (10) antibiotics. The susceptibility profile of the multi-drug-resistant clinical isolates of E. coli and S. aureus on T. procumbens leaf extracts was evaluated using agar well diffusion. Extraction of the plant material was done using methanolic solvent. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to determine the chemical compounds that are present in the plant material. The results obtained shows that total of 63% and 64% of the E. coli and S. aureus respectively, were MDR strains. A total of 25 compounds were identified from the GC-MS analysis of the methanolic fraction of Tridax procumbens. The results obtained from this study suggest that there is misuse and abuse of the screened antibiotics in the population. So, antimicrobial resistance remains a global public health challenge. Methanolic extract of T. procumbens was satisfactorily active against MDR E. coli while MDR S. aureus was comparatively less sensitive