REVALENCE AND ANTIMICROBIAL RESISTANCE PATTERNS OF VIBRIO SPP. AND AEROMONAS SPP. IN PANGASIUS FISH FROM MARKETS IN SIEM REAP, CAMBODIA
Keywords:
Pangasius fish, Vibrio spp., Aeromonas spp., antimicrobial resistance, antibiotic susceptibilityAbstract
Pangasius fish, widely consumed in Cambodia, can harbor pathogenic bacteria such as Vibrio spp. and Aeromonas spp., posing risks to both fish production and public health. The increased use of antibiotics in aquaculture has led to the emergence of antibiotic-resistant bacteria, which are more difficult to manage and control. This study investigated the occurrence and antibiotic resistance profiles of Vibrio parahaemolyticus and Aeromonas hydrophila in 30 Pangasius fish samples collected from three markets in Siem Reap, Cambodia. Samples were handled under strict cold-chain conditions before being analyzed in the Microbiology Laboratory at the Royal University of Agriculture (RUA). Bacterial isolation was performed using selective media (TCBS Agar and MacConkey Agar), followed by purification and identification using Gram staining, biochemical tests (catalase, oxidase, TSI, and motility tests), and confirmation with the API 20E kit. Susceptibility testing was conducted against seven antibiotics using the disk diffusion method. Of the samples tested, V. parahaemolyticus (n = 12, 40%) and A. hydrophila (n = 14, 46.67%) were prevalent. Both isolates exhibited high resistance to Ampicillin and Colistin sulphate but remained highly sensitive to Florfenicol, Sulfamethoxazole/Trimethoprim, and Oxytetracycline. These findings highlight the significant presence of antibiotic-resistant bacteria in Pangasius fish, underlining the need for prudent antibiotic use to reduce the risk of antimicrobial resistance that endangers both aquaculture sustainability and public health
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