ASSESSEMENT OF SOME BIOCHEMICAL MARKERS AND HAEMATOLOGICAL PARAMETERS AMONG DOMESTIC GAS REFILLING ATTENDANTS IN YENAGOA, BAYELSA STATE, NIGERIA.

Abstract

Domestic gas refilling stations are specialists in storing and distributing liquefied petroleum gas. This study focused on assessing some biochemical markers and haematological parameters among domestic gas refilling attendants in Yenagoa, Bayelsa State, Nigeria. Ten millilitre of blood sample was collected via venipuncture technique from twenty seven domestic gas refilling attendants aged 21-32 years with 6-8 years working experience (experimental group) and another twenty seven individuals within the age range of 21-32 years who served as the control group. Five milliliter each of the blood sample was dispensed into lithium heparin and ethylene-diamine-tetraacetic acid anti-coagulated containers respectively with that in the lithium heparin anti-coagulated containers used for the measurement of alanine aminotransferase (colorimetric method), aspartate aminotransferase (colorimetric method), creatinine (Jaffe method), urea (urease berthelot’s method), troponin-1 (dual vial liquid stable immunological method), creatineKinase-MB (immune-inhibition method), C-reactive protein (latex turbidimetric method), interleukin-6 (ELISA method), malondialdehyde (thiobarbituric acid method), glutathione peroxidase (ultra-violet method) and 8-hydroxy-2-deoxyguanosine (ELISA  method ) while the other 5ml of blood in the ethylene-diamine-tetraacetic acid container was used for the measurement of packed cells volume (mico-haematocrit method), haemoglobin (cyanmethaemoglobin method), total white blood cells count (neubauer chamber method), differential white blood cells count: neutrophil count, lymphocytes count, eosinophil count, basophil count, monocytes count (automated haematology analyzer method) and erythrocytes sedimentation rate (westergren method) using SPSS 23.0 version as the statistical package with statistical analysis being students “t” test. The findings in the experimental group individuals indicated no significant differences in the mean values of alanine aminotransferase (11.38 ± 1.43) IU/L, aspartate aminotransferase (10.23 ± 1.37) IU/L, creatinine (72.42 ± 3.73) µmol/L, urea (7.87 ± 1.76) mmol/L, troponin-1 (1.30 ± 0.31) ×10^-2(IU/L), creatineKinase-B (6.94 ± 1.07) IU/L and 8-hydroxy-2-deoxyguanosine  (2.26 ± 0.73) ng/ml when compared to the control group: alanine aminotransferase (11.35 ± 1.40) IU/L, aspartate aminotransferase (10.20 ± 1.36) IU/L, creatinine (72.40 ± 3.71) µmol/L, urea (7.85 ± 1.74) mmol/L, troponin-1 (1.27 ± 0.28) ×10^-2(IU/L), creatineKinase-B (6.92 ± 1.02) IU/L and 8-hydroxy-2-deoxyguanosine  (2.25 ± 0.71) ng/ml. However, the mean values of C-reactive protein (8.02 ± 0.91) mg/L, interleukin-6 (7.72 ± 0.64) pg/ml and malondialdehyde (4.23 ± 0.79) µmol/L were significantly elevated as compared to the control group: C-reactive protein (3.74 ± 0.48) mg/L, interleukin-6 (5.24 ± 0.31) pg/ml and  malondialdehyde (2.16 ± 0.68) µmol/L while glutathione peroxidase (1.87 ± 0.44) µmol/L was significantly reduced as compared to the control group (2.01 ± 0.60) µmol/L. The mean values of the measured packed cells volume (35.00 ± 0.06) %, haemoglobin (11.00 ± 0.03) g/dL and lymphocytes (22.00 ± 0.04) %,were significantly reduced when compared to the control group: packed cells volume (42.00 ± 0.17) %, haemoglobin (13.70 ± 0.08) g/dL and lymphocytes (30.00 ± 0.18) %,while total white blood cells count (13,800 ± 1.47) cmm, neutrophil count (78.00 ± 1.31) %, and erythrocytes sedimentation rate (8.00 ± 0.12) mm/Hour were significantly elevated when compared to the control group: total white blood cells count (9,700 ± 1.21) cmm, neutrophil count (70.00 ± 1.26) %, and erythrocytes sedimentation rate (2.00 ± 0.04) mm/hour. Domestic gas refilling attendants with 6-8 years working experience exhibit a tendency towards inflammatory disorder, oxidative stress disorder, iron deficiency anaemia and infection with no evident adverse effects on liver, renal and cardiac functions.