Aim: This study investigates the level of knowledge and extent of utilization of Information and Communication Technology (ICT) tools in healthcare delivery among health center workers in Port Harcourt Metropolis. Methodology: A cross-sectional descriptive design was adopted, targeting healthcare workers in three universities in Rivers State: University of Port Harcourt (39 respondents), Rivers State University (48 respondents), and Ignatius Ajuru University of Education (42 respondents). A total of 129 participants were selected using convenience sampling. Data was collected using a structured questionnaire titled “Knowledge, Attitude, Practices, and Perceptions of University Health Centers Workers’ Questionnaire (KSAPPQ).” Analysis was performed using SPSS Version 27, employing descriptive statistics, chi-square tests, and regression analysis to assess associations between knowledge, sociodemographic variables, ICT utilization, and barriers. Findings: The results revealed a poor level of ICT knowledge, with an aggregate of 41.39% correct responses, and 58.61% incorrect responses. Only a few areas, such as ICT training (45.7%) and telemedicine (45.7%), showed relatively higher knowledge but remained below satisfactory levels. The mean extent of ICT utilization was 2.70 ± 1.20, indicating a low extent of use. Specific tools like Electronic Health Records (3.32 ± 1.18) and diagnostic systems (3.34 ± 1.16) were moderately utilized. Conclusion: Conclusively, this research thus accomplished that there is a poor level of knowledge of all health worker in universities in Rivers State towards information, communication and technology. It is imperative to sustain and expand its initiatives. (30 words). Recommendation: Health Educators and Health promotors should enhance ICT awareness campaigns: like organize events, create posters, and distribute brochures to raise awareness about the importance of ICT and develop ICT-related content like create blog posts, videos, and social media content to educate users about ICT concepts and best practices