Maize is an important cereal crop grown in the Federal Capital Territory (FCT), Abuja. It has a chromosome number of 2n = 2× = 20. There is little knowledge on the viruses affecting maize within this region. This research was carried out to identify the virus affecting maize on four commonly cultivated maize varieties at Sheda Science and Technology Complex (SHESTCO), FCT, Abuja using a molecular detection tool. Four varieties of maize seeds were obtained from the National Agricultural Seed Council, Abuja and planted on a field using randomized complete block design (RCBD). There was a total of 12 plots and 15 plants per variety per plot. At the fifth week after planting, 15 plants were sampled which gave a total of 60 leaf samples. Maize streak virus (MSV) was confirmed through serological diagnosis, although severity and percentage identification varied. Only maize streak virus was targeted. Polymerase chain reaction (PCR) confirmed the presence of maize streak virus and sequence revealed the strain of the virus and percentage identification. This research provides additional information on the presence of MSV as a threat to maize production in the Federal Capital Territory, Abuja. This information can also be utilized by virologist and breeders to improve varieties of maize that can resist maize streak virus